MVA-C-ΔF1L deletion mutant enhances the production of type I IFNs and cytokines in murine macrophages (A) and dendritic cells (B).

<p>BMDMs or BMDCs were mock-infected (0) or infected with MVA-C or MVA-C-ΔF1L at 1 or 5 PFU/cell for 6 hours (upper panels) or 24 hours (lower panels). IFN-α, IFN-β, IL-1β, TNF, IL-6, IL-12p40 and MIP-1α mRNA (upper panels) and protein (lower panels) levels were quantified by RT-PCR and ELISA, respectively. mRNA results are expressed as the ratio of the gene of interest to <i>Hprt</i> mRNA levels. A.U: arbitrary units. ELISA results are means ± SD of duplicate samples from one experiment and are representative of two experiments. * <i>p</i><0.05 for all conditions comparing MVA-C-ΔF1L to MVA-C at the same MOI.