ECP but not I13A form amyloid-like aggregates on the surface of bacteria and membranes.

<p>(A) <i>E. coli</i> bacteria cells stained with Hoechst (blue signal) were incubated with unlabeled wtECP or I13A mutant for 4 h, stained with ThT (green signal) and visualized by TIRF microscopy. In all experiments cell cultures were grown at exponential phase (OD₆₀₀ = 0.2) and incubated with proteins in 10 mM sodium phosphate buffer, 100 mM NaCl, pH 7.4. (B) Planar lipid bilayers prepared as described in the <i><a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003005#s3" target="_blank">Materials and Methods</a></i> section (stained with DiI; red signal) were incubated with 5 µM of either unlabeled wtECP or I13A mutant for 4 h, stained with 25 µM ThT (green signal) and visualized using a Olympus FluoView 1000 TIRF microscope. Scale bar represents 20 µm (5 µm in the insight caption). Images depicted are representative from two independent experiments.