Viral colocalization with endosomes at early infection.

<p>Representative confocal micrographs of Vero cells infected with ASFV and immunostained for viral capsid proteins p72 and pE120R (shown in red), and in green, EE marker EEA1 (<b>A</b>), MVB marker CD63 (<b>B</b>), LE marker Rab7 (<b>C</b>) and LY marker Lamp1 (<b>D</b>) at 15 minutes postinfection (mpi). Scale bars, 10 µm. Cells were infected at a moi of 10 pfu/cell and adsorption was maintained at 4°C for 90 min. Unbound virus was then washed, cells were shifted to 37°C and infection was allowed to progress for indicated times. (<b>E</b>) Percentages of colocalization events of p72 capsid protein with EE or LE marker are expressed as means and relativized to the total cell-associated virus particles per individual cell at each time point in 10 cells in duplicates. (<b>F</b>) Percentages of colocalization events of p150 inner core protein with LE marker expressed as means and relativized to the total cell-associated virus particles per individual cell at each time point in 10 cells in duplicates. (<b>G</b>) Representative confocal micrograph of the colocalization of viral cores with Rab7 positive endosomes. Nuclei were stained with TOPRO3. (<b>G1–4</b>) Detail of colocalization between viral cores and LE in high magnification of the boxed areas in (G). (<b>H</b>) Colocalization of viral core protein p150 with Lamp1 marker.