Screening for modifiers of polyQ-induced toxicity.

<p>(<b>A</b>) Rough eye phenotype (REP) used as a primary readout for screening. Compared to control (upper panels), eye-specific (<i>GMR-GAL4</i>) expression of polyQ (lower panels) induces disturbances of the external eye texture, e. g. depigmentation of the compound eye observed by light microscopy (left) and as depicted in scanning electron micrographs (middle). Toluidine blue-stained semi-thin eye sections reveal that the disturbance of external eye structures is accompanied by degeneration of retinal cells (right). (<b>B</b>) Modification of the polyQ-induced REP by enhancers and suppressors. VDRC transformants used to silence respective genes: <i>CG3284</i> (11219), <i>CG16807</i> (23843), <i>CG15399</i> (19450) and <i>CG7843</i> (22574). (<b>C</b>) Flow chart of the screening procedures to identify modifiers of polyQ-induced toxicity. (<b>D</b>) Brief summary of screen results. Scale bars represent either 200 µm in eye pictures or 50 µm in semi-thin eye sections.