Western blot analysis of Pten and p-p70S6 kinase (isoform p-p70 of the S6 kinase, indicates mTOR activation) and examination of miR-21 expression by real time PCR a) Pten analysis of MPNST cell lines, dermal fibroblasts and neurofibroma derived Schwann cell cultures (SC1-4).

<p>The grey line indicates mean Pten expression of 4 Schwann cell cultures. b) Quantification of Pten expression (normalized with β-actin). c) Expression of Pten and p-p70S6 kinase in <i>NF1</i> positive (+/+) and <i>NF1</i> negative (−/−) Schwann cells. d) Relative expression of miR-21 as determined by real time PCR. * Endogeneous control RNU44 was not detectable. This cell line was thus omitted from analysis. SC = neurofibroma derived Schwann cells.