quantitative Real-Time RT-PCR experiments to assess fungal RNA contents in mixed transcriptome samples.

<p>The presence of fungal RNA in the mixed transcriptome samples (COM: compatible, INC: incompatible interactions) was examined by qRT-PCR using artificial mixed transcriptome samples at several fungal RNA concentrations (0, 0.5, 1, 3, 5, 10, 20, 30, 40 and 100%). Crossing point (CP) values represent the point at which the amount of the amplified fungal actin reached a fixed threshold. High CP values indicate low abundance of fungal RNAs. The means and standard errors of CP values of three technical replicates are shown for each sample.