Hypercholesterolemia promotes leukocytosis in peripheral blood of LDLr^−/− mice.

<p>Hypercholesterolemic LDLr^−/− mice were used to study the effect of LDL on HSPC. At the age of 8 weeks, mice were placed either on normal diet or on high fat diet for 2 months. Two months after normal or high fat diet, blood cells of LDLr^−/− mice were stained with antibodies against Ly-6c, F4/80, Ly-6G and CD11b. The percentage of Ly-6c^hi monocytes (A), F4/80+ monocytes (B) and Ly-6G^hi granulocytes (C) was quantified by FACS.