β-catenin status, wound healing and stem cell markers during the healing process in small wounds.

<p>Full-thickness wounds (diameter = 0.5 cm) were generated on the backs of 8-week-old C3H mice. Wounded tissues were excised at 1, 4, 7, and 10 d post-wounding, and subjected to H&E staining, immunohistochemical analysis, and western blotting as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048791#s2" target="_blank">Materials and Methods</a>. (A) H&E staining (first row panels) (original magnification ×100) and immunohistochemical staining for β-catenin, keratin 14, α-SMA, collagen I, and collagen III (other row panels) in the wounds (original magnification ×200). EP, epidermis. (B) Western blot analyses of β-catenin, keratin 14, α-SMA, collagen I, collagen III, and ERK in large and small wounds. (C) Immunohistochemical analysis of Nestin or CD34 in the wounds at 1, 4, 7, 10 d post-wounding (original magnification ×635).