LDL increased myeloid cell production in vitro.

<p>Sorted LSK cells of C57BL/6 mice were seeded at 1000 cells per well in 96-well plate and cultured in SFEM supplemented with IL-3, IL-6 and SCF for 14 days. LDL or LDL plus HDL were added as indicated. GM-CSF was used as the positive control. Total cells and cells with morphological features of differentiated cells were enumerated under the microscopy. (A) Data were expressed as the percentage of undifferentiated cells in total cells. Cells were stained with antibodies against CD11b PE and Ly-6c PE-Cy7 (B), CD11b PE and F4/80 APC-Cy7 (C), and CD11b PE and Ly-6G APC (D) for FACS analysis.