Fluorescence staining results for NSPC differentiation.

<p>Quantification of IHC at day 14 shows that more porous scaffolds (up to 20 wt% D-mannitol initially) in (A) neuron specific media (IFN-γ) favor neurons. (B) Oligodendrocyte specific media (PDGF-AA) favor oligodendrocytes and (C) in astrocyte specific media (BMP-2) favor astrocytes. (D) Control media with no growth factors (-GF) as well as with proliferation growth factors (+EGF+FGF) maintain nestin expression (note: error bars are included but too small to see). Letters denote significance by single factor ANOVA (p<0.001). Mean ± SD with n = 3.