Distribution of the CnMV marker and glial cells around <i>C. neoformans</i>-induced cystic lesion in infected brains.

<p>C57LB/6 mice were infected with 10⁶<i>C. neoformans</i> JB02 cells for 10 days. Brain sections were stained with an anti-GFP antibody to detect the CnMV marker 14-3-3-GFP (green) (<b>B</b>), an anti-GFAP (glial fibrillary acidic protein) antibody to detect host astrocyte cells (red) (<b>C</b>), and DAPI for the localization of brain cells (blue) (<b>D</b>). Image (<b>A</b>) is the overlaid image of (B, C, D). Image (<b>E</b>) is a section of an uninfected brain with no detectable staining for GFP. The <i>C. neoformans</i> strain C1186 was used to infect mice for comparison; green yeast cells were evident but CnMVs could not be detected in brain sections (<b>F</b>). A confocal image of a typical cystic lesion after 16 days of infection is shown in (<b>G</b>). Enlarged regions (box 1, 2, and 3) in image (<b>G</b>) are displayed in (<b>1</b>), (<b>2</b>)<b>,</b> and (<b>3</b>), and their corresponding videos are provided in Supplement V(1), V(2), and V(3), respectively. Bar = 50 µm.