UW426 MB cells are sensitive to stem cell-delivered TRAIL.

<p>(a) Plot showing the viability of UW426-Fluc-mCherry MB cells co-cultured with hMSC-GFP cells with different ratios of hMSC to MB cells. (b) Plot showing the viability of UW426-Fluc-mCherry MB cells co-cultured with hMSC-S-TRAIL cells with different ratios of hMSC to MB cells. (c) Plot showing the viability of UW426-Fluc-mCherry cells on 0, 2, 5 days of co-culture with hMSC-GFP or hMSC-S-TRAIL cells. Representative pictures showing UW426 (red) co-cultured with hMSC (green) obtained on day 5. (d) Plot showing Fluc bioluminescence activity of intra-parenchymally implanted mice with UW426-Fluc-mCherry cells with or without hMSC-GFP on days 2, 3, and 5 post-implantation. Representative images are shown from one mouse per group on day 3. (e) Fluc bioluminescence activities of intra-parenchymally implanted UW426-Fluc-mCherry cells with hMSC-GFP or with hMSC-S-TRAIL at 2, 3 and 5 days post-implantation. Representative images are shown from one mouse per group on day 3. (f–q) Photomicrographs and immunohistochemistry of brain sections on day 2 from mice bearing UW426-Fluc-mCherry tumors alone or with hMSC expressing either TRAIL and GFP or GFP. (r) Plot showing the number of cleaved caspase-3-positive UW426 cells in tumors without hMSC or with hMSC-GFP or hMSC-S-TRAIL (Original magnification: c, ×10; f–q, ×20.). * denotes p<0.05 in the comparison of each treatment to controls, student’s t-test.