Prophylactic efficacy of a complex of hH1N1-HK HA233 and Lipoplex(O) against maA/WSN/1933 virus.

<p>BALB/c mice were immunized i.p. two times with a complex of MB-ODN 4531(O) encapsulated in DOPE:CHEMS (Lipoplex(O)), a complex of hH1N1-HK HA233 encapsulated in DOPE:CHEMS (DOPE:CHEMS+hH1N1-HK HA233), or a complex of hH1N1-HK HA233 and Lipoplex(O) (Lipoplex(O)+hH1N1-HK HA233). The immunized mice were challenged intranasally with the maA/WSN/1933 virus. After the virus challenge, the survival rate (<b>A</b>) and the body weight (<b>B</b>) were recorded for 21 days (N = 8/group). Lungs were collected at 3 days or 6 days after the challenge with the maA/WSN/33 virus (N = 3/group) (C). Scale bars in (<b>C</b>), 100 µm. The lung viral titers were measured by means of a plaque assay to estimate the viral clearance at 3 days or 6 days after the challenge with the maA/WSN/1933 virus (<b>D</b>).