Nox1 catalyzes ROS generation associated with BLT2 signaling in MDA-MB-231 and MDA-MB-435 cells.

<p>(A) Semiquantitative RT-PCR analysis of Nox1 and Nox4 mRNAs in MCF-10A, MDA-MB-231, and MDA-MB-435 cells. Data are representative of three independent experiments. (B) MDA-MB-231 and MDA-MB-435 cells were incubated with U75302 (1 µM), LY255283 (10 µM), or DMSO vehicle for 1 h or were transfected with BLT2 or control siRNAs for 48 h before determination of the abundance of Nox1 and Nox4 mRNAs by semiquantitative RT-PCR analysis. Data are representative of three independent experiments. (C and D) MDA-MB-231 and MDA-MB-435 cells were transfected with a vector for Nox1 siRNA (pSUPER-siNox1) or the corresponding empty vector for 48 h and were then analyzed for the intracellular level of ROS (C) or invasiveness (D). Scale bars, 50 µm. Data are expressed relative to the corresponding value for cells transfected with the empty vector and are means±SD from five independent experiments. *<i>P</i><0.05, †<i>P</i><0.01, ‡<i>P</i><0.005.