<p>(<b>A</b>) Size exclusion chromatography of Sso7d mutants purified by immobilized metal affinity chromatography (IMAC). The dashed box indicates elution peak for Sso7d mutants. Mutants were loaded on the column at a concentration of 2 mg/ml. Molecular weight estimates based on the retention time of Sso7d mutants in the column are consistent with the mutants being present in monomeric form. The other peak corresponds to a minor impurity with higher molar absorptivity than the Sso7d mutants (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048928#pone.0048928.s003" target="_blank">Figure S3</a>; SDS-PAGE analysis of fractions corresponding to the other peak do not show any detectable protein). (<b>B</b>) Circular ...
<p>The library obtained by random mutagenesis of a pool of hFc binders was screened using magnetic s...
<p>Size exclusion chromatograms of wild-type Fc and disulfide bond stabilized Fc-mutants monitored a...
<p>Htt is a molecule with elongated shape and mutant Htt128 is more extended than Htt17 and Htt46. (...
<p>(<b>A</b>) Sso7d-hFc, Sso7d-his-hFc and Sso7d-ev-hFc were recombinantly expressed with a C-termin...
<p>(<b>A</b>) End-point assay to determine pH sensitivity for Sso7d-hFc. Yeast cells displaying Sso7...
<p>(<b>A</b>) Yeast surface displayed hFc was subjected to thermal denaturation at 99°C and subseque...
<div><p>We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin ...
We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin G (hIgG)...
<p>(<b>A</b>) End-point assay to determine pH sensitivity for Sso7d-his-hFc. Yeast cells displaying ...
<p>(<b>A</b>) End-point assay to determine pH sensitivity for Sso7d-ev-hFc. Yeast cells displaying S...
<p>Amino acid changes in mutants with single amino acid substitutions that retain binding to Protein...
<p>Mean residue molar ellipticities, in degrees, of A (Blue): G165fs; B (Violet): L45PL54P; C (Maroo...
<p>(A) Size-exclusion chromatography profiles. 100 µl of protein was loaded onto a Superdex 200 10/3...
<p>(A) Size-exclusion chromatography profiles of wild-type HSPB6, Δ31–40 and Δ61–70. 100 µl of prote...
<p>(<b>A</b>) Yeast displayed Sso7d-hFc, Sso7d-his-hFc and Sso7d-ev-hFc were labeled with 1 µM hFc o...
<p>The library obtained by random mutagenesis of a pool of hFc binders was screened using magnetic s...
<p>Size exclusion chromatograms of wild-type Fc and disulfide bond stabilized Fc-mutants monitored a...
<p>Htt is a molecule with elongated shape and mutant Htt128 is more extended than Htt17 and Htt46. (...
<p>(<b>A</b>) Sso7d-hFc, Sso7d-his-hFc and Sso7d-ev-hFc were recombinantly expressed with a C-termin...
<p>(<b>A</b>) End-point assay to determine pH sensitivity for Sso7d-hFc. Yeast cells displaying Sso7...
<p>(<b>A</b>) Yeast surface displayed hFc was subjected to thermal denaturation at 99°C and subseque...
<div><p>We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin ...
We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin G (hIgG)...
<p>(<b>A</b>) End-point assay to determine pH sensitivity for Sso7d-his-hFc. Yeast cells displaying ...
<p>(<b>A</b>) End-point assay to determine pH sensitivity for Sso7d-ev-hFc. Yeast cells displaying S...
<p>Amino acid changes in mutants with single amino acid substitutions that retain binding to Protein...
<p>Mean residue molar ellipticities, in degrees, of A (Blue): G165fs; B (Violet): L45PL54P; C (Maroo...
<p>(A) Size-exclusion chromatography profiles. 100 µl of protein was loaded onto a Superdex 200 10/3...
<p>(A) Size-exclusion chromatography profiles of wild-type HSPB6, Δ31–40 and Δ61–70. 100 µl of prote...
<p>(<b>A</b>) Yeast displayed Sso7d-hFc, Sso7d-his-hFc and Sso7d-ev-hFc were labeled with 1 µM hFc o...
<p>The library obtained by random mutagenesis of a pool of hFc binders was screened using magnetic s...
<p>Size exclusion chromatograms of wild-type Fc and disulfide bond stabilized Fc-mutants monitored a...
<p>Htt is a molecule with elongated shape and mutant Htt128 is more extended than Htt17 and Htt46. (...