Apparent equilibrium dissociation constants (K_D) for the Sso7d mutants.

<p>For each mutant, data from at least two independent experiments were fit globally to a four parameter logistic model using non-linear least squares regression for estimation of K_D. The corresponding 68% confidence intervals are shown in parentheses.</p>a<p>Not determined. The binding isotherm at pH 4.5 did not reach saturation even with 20–30 µM concentration of Sso7d-his-hFc and Sso7d-ev-hFc; consequently, an apparent K_D for the binding interaction at pH 4.5 could not be calculated.