Expression, clustering, and functional classification of genes expressed in human prostate CTCs.

<p>(A) Expression of prostate cancer associated genes. For each gene, fragments per kilobase of exon per million fragments mapped (FPKM) for each CTC and controls are shown. FPKM values greater than 5 are shaded green and those with values between 1 and 5 are shaded light green. AR (androgen receptor), KLK3 (prostate specific antigen), and TMPRSS2are markers of prostate tissue. CD45 is a white blood cell marker. Prostate cancer cell lines include LNCaP and PC-3 while T24 is a bladder cancer, and WBC is a single white blood cell. Normal denotes normal prostate tissue. (B) Unsupervised clustering of over-expressed genes in patient CTCs. Colored bars across the top of the figure indicate different patients while individual patient CTCs are listed at the bottom of the cluster. (C) Functional classification of genes overexpressed in CTC using Gene Ontology (GO) classifications. For each functional grouping the % of genes over-expressed in each GO category is indicated.