RAD21 binding to <i>MYC</i> regulatory elements varies between breast cancer cell lines.

<p>RAD21 binding in MCF7, T47D and MDA-MB-231 cell lines was analyzed by ChIP. RAD21 binding is shown as fold enrichment relative to input chromatin and normalized against a negative site (NEG) where no binding was observed. The bar graph represents the mean +/− SEM of three independent experiments. The symbols * and ** indicate significant differences (p<0.05 and p<0.01 respectively) in RAD21 binding relative to the MCF7 cell line. A schematic of primer locations is shown below the histogram. ChIP primer sequences are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049160#pone.0049160.s004" target="_blank">Table S1</a>. A scale diagram of primer positions relative to the <i>MYC</i> gene and promoters is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049160#pone.0049160.s003" target="_blank">Figure S3</a>.