PF-46396 resistance mutations map to three regions within the CA-CTD and SP1.

<p>[A] Example of a selection experiment for PF-46396 resistance. The Jurkat T-cell line was transfected with pNL4-3 and propagated in the presence of 0–5 µM PF-46396. Virus replication was monitored by RT activity, shown in counts per minute (cpm) per µl of culture medium. [B] Location of PF-46396 resistance mutations in Gag. A linear diagram of Pr55^Gag is provided, with CA N-terminal and C-terminal domains (NTD and CTD, respectively) indicated. Small black boxes denote the location in Gag of the resistance mutations, which are shown below using CA and SP1 amino acid numbers. Single-underlined mutations represent those that we previously obtained during BVM selections; highly conserved residues in the MHR are denoted by double underlines.