MR, GR, 11β-HSD1, 11β-HSD2 expression in iris/ciliary body and corticosterone concentration in aqueous humor during EIU. A–B:

<p>Time-course of MR (A) and GR (B) mRNA expression (real-time PCR). After LPS injection, down-regulation of GR was observed only at 24 hours, whilst MR expression was down-regulated already at 3 hours. The 18S was used as housekeeping gene. Results in EIU are relative to those without LPS injection. Data are means ± SEM; n = 6 rats per conditions; **p<0.01, ***p<0.001. <b>C:</b> Immunohistochemistry of MR and GR in iris sections. In normal iris rat, MR and GR are localized in the nuclei of the iris epithelial cells (arrow), endothelial cells (arrowhead) and probably in other resident cells of the iris stroma. Twenty-four hours after LPS injection, MR and GR expression signals were reduced in uveitis eyes (star: vessels). Negative controls are signals yielded in the absence of primary antibody. Scale bar = 40 µm; 20 µm for insert. <b>D–F:</b> Time-course of 11β-HSD2 (D), 11β-HSD1 (F) expression (real-time PCR) and corticosterone concentration in aqueous humor (E). LPS injection induced an early (3 hours) down-regulation of 11β-HSD2 transcripts and a late up-regulation of 11β-HSD1 transcripts at 24 hours compared to rats without LPS injection. The 18S was used as housekeeping gene. Results in EIU are relative to those without LPS injection. Corticosterone concentration in aqueous humor increases at 3 and 6 hours after LPS injection. Data are means ± SEM; n = 6 rats per conditions; *p<0.05, **p<0.01, ***p<0.001.