HIPK2 overexpression and Galectin-3 knock-down cooperate in sensitizing MNSC cells to apoptosis. A

<p>, Percentage of apoptotic nuclei in MYCN expressing SHEP cells following Gal-3 overexpression (immunoblot, lower panel) and bleomycin treatment. Significant differences in apoptosis fold induction were obtained between Gal-3 and pcDNA (CTR) transfected cells (**p<0.01). <b>B</b>, Gal-3 knock-down was achieved via transient transfection with RNAi duplexes in MNSC SHEP cells (inset). Analysis of bleomycin induced apoptosis is shown as percentage of apoptotic nuclei (upper panel) and p85^PARP positive cells (lower panel). Significant differences in apoptosis fold induction were obtained between Gal-3i and CTRi transfected cells (***p<0.0001). <b>C</b>, HIPK2 overexpression and Gal-3 knock-down were obtained in MNSC SHEP cells (inset) and the effect on apoptosis in basal condition and following bleomycin treatment was analyzed by counting the percentage of apoptotic nuclei (upper panel) and p85^PARP positive cells (lower panel). Significant differences in apoptosis fold induction were obtained in Gal-3i+HIPK2 transfected cells compared either to CTR or to Gal3i transfected cells (*p≤0.05; **p<0.01).