Correlation of flow cytometric and nCounter results.

<p><b>A:</b> Correlation between results from the nCounter technology and flow cytometry for four typical antigens. Flow cytometric results are given as percentage of cells positive for a given antigen, and nCounter results as digital counts (after normalization and background subtraction). The number of depicted data points varies depending on the antigen tested and on the availability of both cytometric and nCounter values for a given sample. Only samples for which both values were measured are shown. Some samples, particularly informative for a particular antigen, were highlighted with dots in different shades and patterns. nBM, normal bone marrow; corr coeff, Pearson’s correlation coefficient. corr coeff >0.393 are statistically significant with p<0.01. <b>B:</b> Correlation between % of leukemic blasts as determined by flow cytometry, and nCounter values for two typical antigens (CD34, CD19).