MR and GR mRNA expression after intravitreal injection of aldosterone or spironolactone in the eye of LPS-injected rats.

<p>EIU was associated to down-regulation of MR and GR (Fig. 1). <b>A–B:</b> MR expression in iris/ciliary body (real-time PCR): In the inflamed eye, intravitreal injection of aldosterone (Aldo) increased MR transcripts at 6 hours (A) and 24 hours (B) in EIU vs vehicle-injected eyes (Veh). Intravitreal injection of spironolactone (Spiro) decreased MR transcripts compared to vehicle-injected rats at 24 hours (B) and had no effect at 6 hours (A). <b>C–D:</b> GR expression in iris/ciliary body (real-time PCR): In the inflamed eye, intravitreal injection of aldosterone increased GR transcripts at 6 hours (C) and 24 hours vs vehicle-injected eyes (D). GR expression was not statistically modified by spironolactone. The 18S was used as housekeeping gene. Results are relative values of aldo/spiro relative to vehicle injection. Data are means ± SEM; n = 5–7 rats per condition; *p<0.05.