TRIM8 positively regulates TNFα induced NF-κB activation.

<p>(A) TRIM8 enhances TNFα induced NF-κB activation. HEK293 cells were transfected with TRIM8 and vector with NF-κB luciferase reporter constructs as described in materials and methods section. NF-κB activation was measured by Dual Glo luciferase assay. (B) TRIM8 induces p65 translocation to nucleus. Graphical representation of the numbers of cells with nuclear p65-GFP in TRIM8 and vector transfected cells as described in materials and method section. (C) TRIM8 positively regulates NF-κB at the level of p65. HEK293 cells were co-transfected with TRIM8, vector and specified gene. NF-κB activity was monitored by Dual Glo luciferase assay as described earlier. TRIM8 significantly enhances RIP1, cIAP1, TRAF2, IKKβ and p65 mediated NF-κB activation. (D) TRIM8 expression enhances p65 mediated NF-κB activation in HeLa cells. p65 overexpression was confirmed by western blotting. (E) TRIM8 knockdown suppresses p65 mediated NF-κB activation in HeLa cells. TRIM8 knockdown was validated by RT-PCR.