Activation of phospho-Chk2 and p21 by resveratrol in MCF-7 cells.

<p>Effects of resveratrol on phospho-Chk2 (A) and on p21 protein levels (B) in MCF-7 cells. The cells were exposed to various concentrations of <i>trans-</i>resveratrol or DMSO (0.5%) for 24 h. The protein levels were determined by western blotting analysis as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048746#s2" target="_blank">Materials and Methods</a>. GAPDH expression was used as a control. The data are representative of 3 independent experiments, and band intensities are graphically represented with each group of images. ^*, <i>p</i><0.05 in comparison with the respective control (Student's <i>t</i>-test).