Actin has a glycosylated isoform in <i>C. elegans</i>.

<p>(A) Glycoproteins containing terminal GlcNAc were purified by first treating N2 larval lysates with both UDP-azido-GalNAc and GalT (+), or with UDP-azido-GalNAc alone (−), then reacting with biotin-alkyne, and finally capturing the biotinylated glycoproteins with streptavidin beads. Biotinylated glycoproteins were visualized by Western blot with avidin:HRP. (B) Eluted glycoproteins were purified as in (A), fractionated by SDS-PAGE, and the gel was stained with Sypro. Peptides shared by ACT-2, ACT-3, and ACT-4 were identified by MALDI-TOF/TOF from the asterisk-marked band, which was exclusive to the (+) lane.