Inhibition of miR-142-3p increases endogenous D1 receptor protein expression in CAD cells.

<p>Representative western blot (<i>A</i>) and cumulative data (<i>B</i>) showing the levels of D1 receptor protein normalized to total protein in non-differentiated (ND) and differentiated (D) CAD cells 48 hours post-transient transfection with 30 nM per well (in 12-well plate) FAM-labeled negative control anti mir (FAM Anti-mir) or mmu-miR-142-3p anti-mir. The D1 receptor undergoes glycosylation and the three bands in the gel different glycosylated forms of the receptor. The bars represent the mean values ± s.e.m. (n = 3). The endogenous D1 receptor protein level was significantly increased in non-differentiated (*, P<0.006) and differentiated (*, P<0.011) CAD cells transfected with miR-142-3p anti-mir compared to cells transfected with control FAM Anti-mir (two-tailed pair-wise Student’s t-test).