<p>Isolated T lymphocytes were labeled with fluorescent conjugated monoclonal antibodies and sorted by FACS Aria into CD8+CD25hi+ cells and CD4+CD25− cells. As an example, pre (A) and post (B) sorted CD8+CD25hi+ cells and CD4+CD25− cells are shown from a MM patient, and their purity is expressed in percentage.</p
<p>(<b>A</b>) CD8 T cells, derived from the PBL from D01 and D03 were sorted with 245V mutated pp65<...
<p>Negatively isolated CD8<sup>+</sup> T cells from a normal donor who was pMHC-Flu M1 positive and ...
(A) Cord blood mononuclear cells were stained with R123 and chased for 3 hours. Following R123 pulse...
<p>Isolated T lymphocytes were labeled with fluorescent conjugated monoclonal antibodies targeting C...
<p>CD4<sup>+</sup> (A) and CD8<sup>+</sup> (B) lymphocytes were isolated from a cell suspension of P...
Regulatory T cells (Tregs) are a population of lymphocytes that exerts suppressive effects upon the ...
(a) Sample purity following negative MACS bead isolation. The left axis illustrates total lymphocyte...
T cells are an essential part of the immune system. They determine the specificity of the immune res...
A. Representative flow cytometric diagrams from splenocytes before (left column) and after (right co...
<p>Single-cell suspensions from spleens of 12-week-old C57BL/6 were made by crushing the spleen thro...
<p>Mononuclear cell sub-populations were gated and representative dot-plots produced showing levels ...
<p>PBMCs stained and captured by FACSDIVA and analyzed FlowJo software. 1–2 million PBMCs were stimu...
<p>Peripheral blood mononuclear cells (PBMC) and granulocytes were obtained by density gradient cent...
<p>CD4+ T cells are first enriched from draining lymph node cells with untouched paramagnetic bead s...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>(<b>A</b>) CD8 T cells, derived from the PBL from D01 and D03 were sorted with 245V mutated pp65<...
<p>Negatively isolated CD8<sup>+</sup> T cells from a normal donor who was pMHC-Flu M1 positive and ...
(A) Cord blood mononuclear cells were stained with R123 and chased for 3 hours. Following R123 pulse...
<p>Isolated T lymphocytes were labeled with fluorescent conjugated monoclonal antibodies targeting C...
<p>CD4<sup>+</sup> (A) and CD8<sup>+</sup> (B) lymphocytes were isolated from a cell suspension of P...
Regulatory T cells (Tregs) are a population of lymphocytes that exerts suppressive effects upon the ...
(a) Sample purity following negative MACS bead isolation. The left axis illustrates total lymphocyte...
T cells are an essential part of the immune system. They determine the specificity of the immune res...
A. Representative flow cytometric diagrams from splenocytes before (left column) and after (right co...
<p>Single-cell suspensions from spleens of 12-week-old C57BL/6 were made by crushing the spleen thro...
<p>Mononuclear cell sub-populations were gated and representative dot-plots produced showing levels ...
<p>PBMCs stained and captured by FACSDIVA and analyzed FlowJo software. 1–2 million PBMCs were stimu...
<p>Peripheral blood mononuclear cells (PBMC) and granulocytes were obtained by density gradient cent...
<p>CD4+ T cells are first enriched from draining lymph node cells with untouched paramagnetic bead s...
<p>The proportions of CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells and Tregs in PBMCs and in pan...
<p>(<b>A</b>) CD8 T cells, derived from the PBL from D01 and D03 were sorted with 245V mutated pp65<...
<p>Negatively isolated CD8<sup>+</sup> T cells from a normal donor who was pMHC-Flu M1 positive and ...
(A) Cord blood mononuclear cells were stained with R123 and chased for 3 hours. Following R123 pulse...