Neutralization of Tat/Env complex entry or infection in MDDCs by sera from Tat-vaccinated HIV-infected individuals.

<p>(A) Percentage of MDDCs internalizing Env in the presence of sera from trial subjects (n = 7) at baseline (week 0, anti-Tat Ab negative, in blue) and after Tat vaccination (week 48, anti-Tat Ab positive, in red) in the absence (empty bars) or in the presence (filled bars) of Tat. The codes of the sera are indicated at the bottom of the bars. (B) Geometric mean (GM) of the ratio (week 48 vs baseline), with 95% confidence interval (CI) of the percentage of MDDCs internalizing Env in the presence or in the absence of Tat in trial subjects (n = 7). Statistical analysis was performed by the two-tailed Student’s <i>t</i>-test. (C) MDDC infection performed in duplicate for 48 h with pSF162LUC in the presence of coated cys₂₂ Tat (0.01 µM) or BSA (control) with sera from a representative trial subject before (baseline, anti-Tat Ab negative) and after Tat vaccination (anti-Tat positive). Sera prior to or after vaccination contained the same anti-Env Ab titers. Data are expressed as RLU.