The genome-wide distribution of 14 bp EcRE consensus motif in the silkworm.

<p>(A) The location of the genomic sequences identical to 64 patterns of 14 bp consensus motif (5′-KCRGGTCWWCGMWC-3′, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049348#pone-0049348-g007" target="_blank">Fig. 7B</a>). The arrows show the location of 138 motifs in each linkage group of the silkworm. The chromosomal locations of 12 motifs are not certified. Each grey bar shows the scaffold composed of each chromosome. The number located left side of grey bars represents the scaffold number. The white regions show the gap between scaffolds. Underlined numbers on the right of grey bars represent the location for 20E activated genes (more than 4 fold induction by 20E) identified by microarray analysis. 3 and 3-2 are located near the same 14 bp consensus motif. (B) The 20E-dependent activation of predicted-genes surrounding 14 bp consensus motifs analyzed by microarray. The relative expression patterns of 18 genes in aff3 cells treated with 20E for 0, 3, 12, 24 h are shown, based on the spot intensities for the predicted genes in the silkworm 44K cDNA microarray. Detailed information for 18 genes and 17 motifs is summarized in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049348#pone.0049348.s013" target="_blank">Table S6</a>.