Expression of cell type-specific markers at each differentiation step.

<p>qRT-PCR analysis of the expression of pluripotency markers (A: <i>Oct3/4</i> and <i>Nanog</i>), endodermal and mesodermal markers (B: <i>Afp</i> and <i>Gata4</i>, respectively), and neural markers (C: <i>Sox1</i> and <i>Pax6</i>) in ESCs, EBs and ESC-derived neurospheres. Fetal cell-derived (ganglionic eminence (GE), cortex and spinal cord) neurospheres and fetal tissues (heart and liver) were used as positive and negative controls, respectively. Data are presented as the means ± SEM (<i>n</i> = 3).