RpRalF inefficiently restores Arf1 recruitment to vacuoles containing <i>Legionella ΔralF</i>.

<p><b>A</b>) Representation of the constructs used in the complementation experiment. <b>B</b>) HEK293 cells stably expressing Arf1-GFP were infected with different strains of <i>L. pneumophila</i> (wt, <i>ΔralF</i> mutant and <i>ΔralF</i> mutant complemented with M45-LpRalF, M45-LpRalF_E103A, M45-RpRalF_1–342SS or M45-RpRalF_{1–342SSE100A},). Cells were fixed 1 h post-infection, extracellular bacteria were stained in blue, and total bacteria in red. Bar = 1 µm. <b>C</b>) Quantification of Arf1-GFP recruitment to the LCV by the indicated <i>L. pneumophila</i> strains. Represented is the average of 3 experiments where 50 vacuoles were counted. Standard deviations are indicated.