Spry1 null CD4⁺ T cells have enhanced effector function.

<p>Spry1 null and wild type spleens were expanded <i>in vitro</i> with anti-CD3; CD4⁺ T cells were isolated and rechallenged with increasing doses of anti-CD3. <b>A.</b> IL-2 production by CD4⁺ T cells was determined by ELISA. <b>B.</b> CFSE dilution of CD4⁺ wild type and Spry1 null CD4⁺ T cells. <b>C.</b> IFNγ production was determined by intracellular cytokine secretion assay. % of Max indicates the fraction of the total cells at a given fluorescent intensity. <b>D.</b> Following expansion Spry1 null and wild type spleens were stimulated with increasing doses of ionomycin, briefly rested and rechallenged with anti-CD3. Resistance to ionomycin-induced anergy is displayed as percentage of IL-2 produced following ionomycin treatment and rechallenge compared to rechallenged alone. Error bars represent one standard deviation of the mean. P values indicate statistical significance by student t-test. All experiments were performed at least three times. Data are representative of 3 mice per group.