Decreased brain apoptosis and neuroinflammation in 720-day-old male rats previously exposed to neonatal hypoxia.

<p>(<b>A</b>) Apoptotic cells in various key brain areas analyzed by means of the Apostain F7-26 specific monoclonal antibody against single-stranded DNA. Data are expressed as number of positive cells per mm³ (means ± s.d.) and were obtained from 3 individuals in each group. Statistically significant differences between control and hypoxia groups: *<i>P</i><0.05, **<i>P</i><0.01. (<b>B</b>) Illustration in the prefrontal (PF) cortex. (<b>C</b>) DAP kinase mRNA levels in various brain structures, with a significant decrease in the neurogenic subventricular zone (**<i>P</i><0.01). (<b>D</b>) Noticeable hypoxia-associated decreased neuroinflammation in the CA1 layer and in the prefrontal cortex in male rats belonging to the hypoxia group, as shown by the use of Ox-6 antibody (cells were counterstained by Dapi and the neuronal marker NF68).