Levels of IL-10 mRNA, intracellular IL-10 production and SOCS expression

<p>(<b>A</b>)<b>.</b> Levels of IL-10 mRNA expression in lymph nodes at day 42 in LNT-GFP or LNT-IL-10 mice. (<b>B</b>) The amount of IL-10/cell measured as geometric mean flourescent intensity (MFI) in lymph node CD19⁺MHC II⁺B cells, (<b>C</b>) in lymph node CD19^-MHC II⁺non-B APCs (<b>D</b>) in splenic B cells, (<b>E</b>) in splenic non-B APCs. (<b>F</b>) Typical gating for intracellular cytokine staining showing one sample from an LNT-GFP mouse and an LNT-IL-10 mouse (<b>G</b>) Levels of mRNA SOCS1 and 3 expression in draining lymph nodes at day 42. In <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049731#pone-0049731-g002" target="_blank">figure 2A–E and G</a> data were analysed by Mann-Whitney U-test. Closed circles represents LNT-GFP and open circles LNT-IL-10 mice.