Effects of neonatal hypoxia on synapsin phosphorylation in 720-day-old male rats.

<p>(<b>A–D</b>) <i>In situ</i> coupling between synapsins with phospho-Ser⁹ and phospho-Tyr³⁰¹, as monitored by the Duolink® “proximity ligation” assay: number of dots per cell in CA1 and frontal cortex (Cx) of control and hypoxic male rats. Experiments were performed in triplicate (*<i>P</i><0.05, **<i>P</i><0.01). (<b>E–G</b>) Illustrations of <i>in situ</i> association between synapsin I and phospho-Ser⁹ and phospho-Tyr³⁰¹ in the CA1 layer and the dentate gyrus (DG, panel G) of control and hypoxic rats. Cells were counterstained with Dapi and NeuN.