<p>A) Cells treated with dsRNA against 2 different regions DmRecQ4 (N terminal – NRecQ4 and C terminal – CRecQ4) or against a control dsRNA were fixed 4 days after dsRNA treatment and subjected to immuno-fluorescence microscopy using antibodies against Drosophila phospho-H2AvD. The DNA was visualised with DAPI. B) Chromatin was prepared from untreated S2 cells (no dsRNA) cells treated with a control dsRNA (control dsRNA) or cells treated with dsRNA against the N terminal region of DmRecQ4 (RecQ4 dsRNAi 4 days after dsRNA treatment and analysed by western blotting using an anti phospho-H2AvD antibody (top panel). This detects both phosphorylated H2AvD (*) and H2a (**). The etoposide control lane is included to allow accurate identification o...
(A) Recombinant H2Av-Flag (rH2Av-Flag) was transfected into S2 cells to make a stable cell line usin...
<p>(A) Results of the immunocytochemical analysis and the method of <i>tissue printing</i>. (a-a', b...
<p>(A–B) Live analysis of salivary gland nuclei of late third-instar <i>UAS-LacZ/+; ey-GAL4/+</i> (U...
<p>A) Total extracts of S2 cells which had been mock depleted (control) or depleted of DmRecQ4 by ds...
<p>Chromatin was prepared from untreated S2 cells, cells that had been treated with HU for 24 h and ...
<p>A) Diagrammatic representation to show the deletions of the SLD2 homology domain, the Drosophila ...
<p>A) Immunofluorescence analysis shows increased numbers of SG4 cells exhibiting γ-H2AV foci follow...
<p>Western Blot analysis of whole S2 cells histone extracts or protein extracts to analyze either le...
<p>(A) Although the majority of TPX2 is found in the soluble fraction (see <a href="http://www.ploso...
The recognition of DNA double-strand breaks (DSBs) using a phospho-specific antibody to the histone ...
<p>(A) Immunostaining for endogenous SRSF2 in HeLa cells untreated or treated with TSA (0.2 ng/µl, 6...
<p>(A) Imaging-based analysis of BMP signals in <i>Drosophila</i> S2 cells. Dpp, Scw and Flag-tagged...
<p>(<b>a</b>) In the WCL of two representative CCDC6-depleted HeLa clones (shCCDC6 #1 and #2) and co...
<p>(A)Chromatin remodeling after S1, S5 or S9 dsRNA transfection. Significant reduction in MNase dig...
<p>HeLa cells were mock infected or infected with WT HSV-1 KOS at an MOI of 10. DRB stocks were prep...
(A) Recombinant H2Av-Flag (rH2Av-Flag) was transfected into S2 cells to make a stable cell line usin...
<p>(A) Results of the immunocytochemical analysis and the method of <i>tissue printing</i>. (a-a', b...
<p>(A–B) Live analysis of salivary gland nuclei of late third-instar <i>UAS-LacZ/+; ey-GAL4/+</i> (U...
<p>A) Total extracts of S2 cells which had been mock depleted (control) or depleted of DmRecQ4 by ds...
<p>Chromatin was prepared from untreated S2 cells, cells that had been treated with HU for 24 h and ...
<p>A) Diagrammatic representation to show the deletions of the SLD2 homology domain, the Drosophila ...
<p>A) Immunofluorescence analysis shows increased numbers of SG4 cells exhibiting γ-H2AV foci follow...
<p>Western Blot analysis of whole S2 cells histone extracts or protein extracts to analyze either le...
<p>(A) Although the majority of TPX2 is found in the soluble fraction (see <a href="http://www.ploso...
The recognition of DNA double-strand breaks (DSBs) using a phospho-specific antibody to the histone ...
<p>(A) Immunostaining for endogenous SRSF2 in HeLa cells untreated or treated with TSA (0.2 ng/µl, 6...
<p>(A) Imaging-based analysis of BMP signals in <i>Drosophila</i> S2 cells. Dpp, Scw and Flag-tagged...
<p>(<b>a</b>) In the WCL of two representative CCDC6-depleted HeLa clones (shCCDC6 #1 and #2) and co...
<p>(A)Chromatin remodeling after S1, S5 or S9 dsRNA transfection. Significant reduction in MNase dig...
<p>HeLa cells were mock infected or infected with WT HSV-1 KOS at an MOI of 10. DRB stocks were prep...
(A) Recombinant H2Av-Flag (rH2Av-Flag) was transfected into S2 cells to make a stable cell line usin...
<p>(A) Results of the immunocytochemical analysis and the method of <i>tissue printing</i>. (a-a', b...
<p>(A–B) Live analysis of salivary gland nuclei of late third-instar <i>UAS-LacZ/+; ey-GAL4/+</i> (U...