Invadopodia and cluster merging in the HA hydrogel invasion assay depend on treatment with motogenic factors.

<p>Images of C4-2 cells cultured for three or six days with either 2% TCM™ (control) or 5% FBS (motogenic factor) in the HA hydrogel invasion assay. Arrow and inset show a magnified view of the image to better display invadopodia. Black scale bars represent 200 µm, white scale bar on inset represents 50 µm (A). Quantification for average number of invadopodia (B) or percent merging clusters (C) in each imaged field. Error bars = SEM, n = 3, ***p<0.001.