Detection of HCV proteins by immunofluorescence in MDBK and BT cells.

<p>The cells infected with liver homogenates from domestic (DR), wild (WR) rabbits and a hare (H) were analyzed 14 days post infection. MDBK cells incubated with MAbs specific for NS3 (A to D), NS4A (E to H) and NS5 (I to L) proteins. BT cells incubated with MAbs specific for NS3 (M to P), NS4A (Q to T) and NS5 (U to X) proteins. Negative controls (NC) for each cell culture and each Mab was also performed (A, E, I, M, Q and U). No fluorescence was detected. Scale bars: (A to H), 20 µm; (I to X), 50 µm.