The bacterial degradation of the nematicide 1,3-dichloropropene, an isomeric mixture, requires the action of <i>trans</i>- and <i>cis</i>-3-chloroacrylic acid dehalogenase (CaaD and <i>cis</i>-CaaD, respectively). Both enzymes are tautomerase superfamily members and share a core catalytic mechanism for the hydrolytic dehalogenation of the respective isomer of 3-haloacrylate. The observation that <i>cis</i>-CaaD requires two additional residues raises the question of how CaaD conducts a comparable reaction with fewer catalytic residues. As part of an effort to determine the basis for the apparently simpler CaaD-catalyzed reaction, the kinetic mechanism was determined by stopped-flow and chemical-quench techniques using a fluorescent mutant f...
Isomer-specific 3-chloroacrylic acid dehalogenases function in the bacterial degradation of 1,3-dich...
The enzymes trans-3-chloroacrylic acid dehalogenase (CaaD) and cis-3-chloroacrylic acid dehalogenase...
The enzymes trans-3-chloroacrylic acid dehalogenase (CaaD) and cis-3-chloroacrylic acid dehalogenase...
The gene encoding the cis-3-chloroacrylic acid dehalogenase (cis-CaaD) from coryneform bacterium str...
The gene encoding the cis-3-chloroacrylic acid dehalogenase (cis-CaaD) from coryneform bacterium str...
trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid to malonate semia...
textThe tautomerase superfamily (TSF) provides an excellent model system to study enzyme specificity...
<i>cis</i>-3-Chloroacrylic acid dehalogenase (<i>cis</i>-CaaD) catalyzes the hydrolytic dehalogenati...
Abstracttrans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of tr...
trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-ha...
The enzymatic conversion of cis- or trans-3-chloroacrylic acid to malonate semialdehyde is a key ste...
textThe tautomerase superfamily is a group of structurally homologous proteins characterized by a [b...
Trans-3-chloroacrylic acid dehalogenase (CaaD) is a critical enzyme in the trans-1,3-dichloropropene...
The enzymatic conversion of cis- or trans-3-chloroacrylic acid to malonate semialdehyde is a key ste...
<i>cis</i>-3-Chloroacrylic acid dehalogenase (<i>cis</i>-CaaD) from <i>Pseudomonas pavonaceae</i> 17...
Isomer-specific 3-chloroacrylic acid dehalogenases function in the bacterial degradation of 1,3-dich...
The enzymes trans-3-chloroacrylic acid dehalogenase (CaaD) and cis-3-chloroacrylic acid dehalogenase...
The enzymes trans-3-chloroacrylic acid dehalogenase (CaaD) and cis-3-chloroacrylic acid dehalogenase...
The gene encoding the cis-3-chloroacrylic acid dehalogenase (cis-CaaD) from coryneform bacterium str...
The gene encoding the cis-3-chloroacrylic acid dehalogenase (cis-CaaD) from coryneform bacterium str...
trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid to malonate semia...
textThe tautomerase superfamily (TSF) provides an excellent model system to study enzyme specificity...
<i>cis</i>-3-Chloroacrylic acid dehalogenase (<i>cis</i>-CaaD) catalyzes the hydrolytic dehalogenati...
Abstracttrans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of tr...
trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-ha...
The enzymatic conversion of cis- or trans-3-chloroacrylic acid to malonate semialdehyde is a key ste...
textThe tautomerase superfamily is a group of structurally homologous proteins characterized by a [b...
Trans-3-chloroacrylic acid dehalogenase (CaaD) is a critical enzyme in the trans-1,3-dichloropropene...
The enzymatic conversion of cis- or trans-3-chloroacrylic acid to malonate semialdehyde is a key ste...
<i>cis</i>-3-Chloroacrylic acid dehalogenase (<i>cis</i>-CaaD) from <i>Pseudomonas pavonaceae</i> 17...
Isomer-specific 3-chloroacrylic acid dehalogenases function in the bacterial degradation of 1,3-dich...
The enzymes trans-3-chloroacrylic acid dehalogenase (CaaD) and cis-3-chloroacrylic acid dehalogenase...
The enzymes trans-3-chloroacrylic acid dehalogenase (CaaD) and cis-3-chloroacrylic acid dehalogenase...