The lAL PNs are produced in a specific birth order.

<p>(A) The table shows the percentage (numbers in the table body) of clones labeling individual PN types (listed in the header column) in all the clones induced at a specific developmental time (indicated as hours after larval hatching [h ALH] in the header row). Only the boxes with the percentage of clones equal to or above 10% are filled with blue. The PNs paired with different types of LNs are separated and put in different rows; for example, “DA1+lavish (LN)” and “DA1+patchy (LN)” are put in separate rows. (B–M″) The <i>GAL4-GH146</i>-labeled ts-MARCM neuroblast (NB) clones of the lAL lineage induced at different developmental times to reveal the birth order of the classical monoglomerular projection neurons (mPNs). The clones are arranged by their birth order from early to late. The single-cell side of the ts-MARCM clone is pseudocolored in magenta, and the NB side of the clone is pseudocolored in green. Brains were co-labeled with nc82 mAb (cyan; B′–M″). The dendritic elaboration in AL (B–M′) and the axonal terminals in LH (B″–M″) are shown separately. The background clones are indicated by arrows. Note the sequential loss of earlier born neurons from the green NB clones. Scale bar: 20 µm.