Mean lipid droplet volume and volume-% of large-sized lipid droplets of dDR larvae and adult <i>C. elegans</i>.

<p>Mean LD volume (µm³) (A–C) and percentage volume of large-sized LDs (>50 µm3) on the total volume of all detected droplets (%) (D–F) within pharynx and tail region was quantified in L2 and L4 larvae and adult wild-type worms. Animals were fed on AL or two different dDR conditions (dDR1.5, dDR0.7). LDs were visualized by fixative BODIPY 493/503 staining. LD volumes were calculated from z-stacks of CLS microscopy images. Eight to ten animals were analyzed per condition and experiment. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046198#s2" target="_blank">Results</a> are shown as mean ± SEM from three independent experiments (*p<0.05, **p<0.01, ***p<0.001).