Schematic of plasmids incorporating the RBS switching strategy in different cloning constructions.

<p>Plasmids for gene over-expression (pDNB101), bacterial two-hybrid system (pDNB102 and pDNB103), bacterial one-hybrid system (pDNB104 and pDNB105) and promoter bank construction (pDNB106 and pDNB107) are presented. Each key element in the vector is labeled and a strong terminator upstream of the <i>cat</i> reporter gene in each of pDNB105, pDNB106 and pDNB107 is shown as a hairpin structure. The <i>Xcm</i>I cassette in each plasmid is shown with grey background.