Cytoskeletal alignment to silk substrate topography.

<p>Fluorescent microscopy images of HCLE cells on (A) glass, (B) flat silk, and (C) patterned silk surfaces (nuclei - blue, actin – red, and vinculin - green, scale bars = 20-µm). (D) Quantification of intracellular to cellular periphery FA formations as indicated by vinculin staining. Fluorescent images and corresponding line-scan analysis of actin fiber distribution of HCLEs cultured on (E) flat and (F) patterned silk film surfaces, where the inset white line of each image indicates region of analysis.