CCHCR1 affects the expression of cytokeratins.

<p>(<b>A, B</b>) The overall expression of cytokeratins is reduced especially in the Iso3Risk (Iso3R) overexpressing cells shown by (<b>A</b>) immunofluorescence staining (here presented vector control, Iso1Non-risk (Iso1N) and Iso3Risk (Iso3R) cells) and (<b>B</b>) Western blotting with pan-cytokeratin (CK) antibody (green). The KRT17 expression is increased in the Iso1Non-risk cells (green). The up-regulation of KRT17 is shown by (<b>A</b>) immunofluorescence (here presented vector control, Iso1Non-risk and Iso3Risk cells), (<b>B</b>) Western Blotting and (<b>C</b>) qPCR. (<b>B</b>) EGF stimulates KRT17 expression in all the other, except the isoform 3-expressing cells. The silencing of CCHCR1 in HEK293 cells (shRNA) downregulates KRT17 expression, shown by (<b>B</b>) Western blotting and (<b>C</b>) qPCR. (<b>B</b>) The expression of KRT17 under CCHCR1 silencing is not restored by EGF stimulation. (<b>B</b>) Also the overall expression of cytokeratins is decreased in CCHCR1 silenced HEK293 cells demonstrated with pan-cytokeratin (CK) antibody. Wt, non-transfected HEK293 cells; shRNA, HEK293 cells transfected with CCHCR1-shRNA construct; Scr, HEK293 transfected with the scrambled sequence. Staining with an antibody for GAPDH was used to control sample loading on SDS-PAGEs. DAPI stained nuclei are shown in blue. Scale bar 10 µm.