Zfp521 suppresses Zfp423 expression.

<p>(A) 3T3-L1 preadipocytes were transduced with retrovirus expressing sh521, shScr, Zfp521, or empty vector. After puromycin selection, RNA was collected and submitted for analysis using Affymetrix arrays. The Venn diagram shows the number of genes up-regulated by sh521 (sh521/shScr>1.5-fold) and down-regulated by Zfp521 (Zfp521/pMSCV<0.7-fold). The heat map corresponds to genes in the intersecting set. (B) Overexpression of Zfp521 in C3H10T1/2 cells represses Zfp423 expression by Q-PCR. (C) Knockdown of Zfp521 in C3H10T1/2 cells enhances Zfp423 expression by Q-PCR. Data presented as mean ± SD, <i>n</i> = 3, *<i>p</i><0.05.