Detection of <i>Sox2</i>-MB in differentiated mES cells.

<p>(A) mES cells stained for SSEA-1 together with the <i>Sox2</i>-MB (blue dots) and the nonspecific-MB (red dots). (B) SSEA-1 stained differentiated mES cells treated with <i>Sox2-</i>MB (blue dots) were compared to SSEA1 stained undifferentiated mES treated <i>Sox2-</i>MB (red dots). (C) Undifferentiated mES cells and mES cells differentiated by exposure to RA were analyzed with RT-PCR. (D) Four quadrants (Q1, Q2, Q3 and Q4) of the differentiated mES cells were selected by comparing the nonspecific-MB fluorescent signal with the <i>Sox2</i>-MB fluorescent signal. (E) The double-positive sorted cell populations (Q2: Sox2-MB⁺ and SSEA1⁺) formed significantly more undifferentiated colonies compared to the positive-negative sorted cell populations (Q1: Sox2-MB^- and SSEA1⁺ Q4: Sox2-MB⁺ and SSEA1⁻ ), and the double-negative sorted cell population (Q3: Sox2-MB^- and SSEA1⁻). (F) Undifferentiated colonies were positively stained for Sox2, Nanog and SSEA1 (Scale bar = 200 µm). Error bars represent the mean ± SEM. Asterisks denotes statistical significance (n = 3 samples ^**<i>p</i><0.01, n = 4 samples^***<i>p</i><0.001).