ATXN2L is found in association with PABP, DDX6 and ATXN2. A)

<p>Scheme of the domain architecture of ATXN2L and ATXN2 highlighting conserved functional motifs. Lines below indicate antibody epitopes for anti-ATXN2L and anti-ATXN2 (BD Biosciences). <b>B)</b> Cell lysates were prepared from HEK293T and HeLa cells as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050134#s4" target="_blank">Materials and Methods</a>. Co-immunoprecipitation experiments were carried out with an anti-ATXN2L antibody, and precipitated proteins were detected using specific antibodies against PABP, DDX6 or ATXN2 (BD Biosciences). <b>C)</b> HeLa cells were fixed and ATXN2L or ATXN2 were stained with an anti-ATXN2L antibody or an anti-ATXN2 antibody (Sigma, red). SR-splicing proteins were stained using an anti-SR antibody (green). Nuclei were visualized by Hoechst staining (blue). Scale bars represent 20 µm.