Genetic organization and transcription analysis of the genomic region including PA2873 <i>locus</i> (<i>tgpA</i>).

<p>(A) PA2875-2874-2873-2872 gene cluster is represented according to visualization by GBrowse in Pseudomonas Genome Database. Locations of fragments amplified by the oligo pairs (Roman numbers) used in RT-PCR-based transcription analysis (B) are shown along the region map. The position of plasmid pDM4 cointegration in PAO1 PA2875::pDM4 strain is indicated. (B) Total RNA was extracted from PAO1 and PAO1 PA2875::pDM4 cells in both exponential (E) and stationary (S) phases, and analyzed by RT-PCR. RT untreated samples (RT−) as controls of genomic DNA contamination were included in the analysis.