<i>rb1</i> mRNA overexpression rescues optic nerve hypoplasia in <i>rb1^te226</i> mutants.

<p>(A–B) Optic nerves of uninjected wild type (A) and <i>rb1^te226a; ath5:gfp^+/−</i> embryos (B) at 48 hpf. (C–D) Optic nerves of <i>rb1^te226a; ath5:gfp^+/−</i> embryos injected with 10 pg of wild type <i>rb1</i> (C) or <i>rb1^te226a</i> (D) mRNA. Dorsal view of maximum intensity projection of confocal z-stacks. (E) Mean optic nerve diameter, measured at dashed rectangle in (A). Error bars denote SEM. *p<0.001, #p<0.01; one-way ANOVA with brackets indicating compared groups. N optic nerves shown at base of bar graphs. Anterior to the top of panel, scale bar = 50 µm.